Technology

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CNTs are a new type of nanomaterial that is attracting attention as gene therapy vectors due to their unique advantages such as large aspect ratio, good biocompatibility, superior mechanical properties, and ease of functionalization. Despite their promising advantages, the use of primitive CNTs in biomedical applications has been limited by their highly hydrophobic surface, which results in poor water solubility and dispersion. Recent studies have shown that CNTs can be surface modified or functionalized using a variety of physicochemical means, making them a new and ideal gene transfer vector. CNTs can be used as excellent carriers for plasmid DNA, small interfering RNA (siRNA), and microRNA with different types of functionalized modifications (e.g. polycations).

Gene therapy can be adapted to each individual to treat a wide range of diseases including cancer and rare diseases. The development of suitable gene transfer vectors to introduce exogenous genes into target cells is of great importance. Currently, research in gene therapy vectors has developed from viral vectors such as retroviruses, adenoviruses, and adeno-associated viruses to today's novel vectors such as baculovirus vectors, which provide a relatively safe, scalable, and cost-effective gene transfer strategy for gene therapy. The baculovirus gene delivery system is an easily modifiable gene therapy system that has been reported to enable site-specific delivery, mitigate adverse effects and improve therapeutics, with the potential to be the cost-effective and efficient backbone for gene therapy. Although challenges remain for recombinant baculoviruses in gene therapy, their high DNA-carrying capacity, maneuverability, and ability to express multiple exogenous genes simultaneousl

Viral vectors have shown great promise in various areas of gene therapy and have led to major breakthroughs in the development of therapies for rare diseases such as severe combined immune deficiency (SCID-X) and hemophilia. Currently, adeno-associated virus (AAV), adenovirus, and lentiviral vectors are frequently used. However, the less commonly used alphavirus vectors offer interesting properties for gene therapy applications and are expected to contribute to the development of gene therapies for rare cancers. Alphaviruses are a class of RNA viruses that can replicate in large numbers within the cytoplasm of the host cell. The replacement of structural protein genes with exogenous genes in an alphavirus vector still has many of the biological properties of an alphavirus such as a broad host spectrum of infection, the ability to self-replicate, and induce apoptosis in transfected cells, while being able to express exogenous genes in large numbers and not integrating easily with the

With the enhancement of environmental awareness, it has become a general trend to replace liquid acid base catalyst with solid acid base catalyst. Compared with liquid catalyst, solid catalyst has advantages such as stronger separation performance, fewer reaction by-products, less corrosion, more environmental protection, and continuous production. As an acidic or basic functional polymer, ion exchange resin has been widely used in esterification, hydrolysis, condensation, dehydration, carbonylation, hydrogenation and other reactions. Different types of resins are available as commercial catalysts, and their differences in polymer matrix, functional groups and final reaction behavior determine their catalytic activity and selectivity. Factors to be considered in selecting catalyst resins include resin type, temperature, dry weight capacity, catalyst porosity, and catalyst expansion/contraction. Amerigo Scientific offers catalyst resins that often used in chemical and petrochemica

Physicians and scientists face the challenge of keeping organs, tissues, and cells viable during various transportation and storage processes for research needs. General products on the market are designed to keep organs, tissue samples, and cells alive for up to 24 hours. Amerigo Scientific offers a solution to preserve tissues, organs, and cells for a longer period for the benefit of physicians and scientists around the world. T-STORE® is a series of sterile, chemically defined, and optimized media that provide a stable storage and shipping environment for mammalian tissue samples and cells. The composition of the medium used to nurture the samples during the preservation protocol is very important, as many factors can negatively affect the integrity of proteins, DNA and RNA in tissues or cells. T-Store® utilizes a robust buffering system, allowing its use over a wide temperature range (+2 °C to +37 °C). In addition, T-Store® is isosmotic, isotonic and isoionic with human serum an

Nucleoside phosphoramidites are derivatives of natural or synthetic nucleosides that are used to synthesize oligonucleotides, relatively short fragments of nucleic acid and their analogs. Oligonucleotides are widely used in biological research as effective tools to regulate gene expression and have also been developed as therapeutics. Therapeutic oligonucleotides are usually modified to enhance their stability and specificity and to minimize the side-effects. They include antisense oligonucleotide, small interference RNA (siRNA), ribozyme (RNA enzymes) and deoxyribozymes (DNA enzymes), CpG oligodeoxynucleotides (CpG ODN), transcription factor decoys, and aptamers. In various oligonucleotide synthesis methods, the phosphoramidite method is the most preferred one as it best meets the criteria for viable synthesis of oligonucleotides. The synthesis approach is suitable for the synthesis of long oligonucleotides with modified backbones, which are difficult to prepared by other methods.

Available Models of Common Age-Related Diseases Bone disease. For example, age-related bone loss, and age-related osteoporosis models. Cardiovascular diseases. For example, the primary hypertension animal model, myocardial infarction animal model, heart failure animal model, etc. Aging lung. Neurodegenerative disease. For example, models of Parkinson's disease and Alzheimer's disease. Multi-morbidity and frailty. For example, some chronic disease models include arthritis, diabetes, cancer, heart disease, and stroke. There are also frailty animal models.

Aging is a biological process that occurs in each of us as individuals and is accompanied by a variety of changes. At the molecular level, DNA methylation occurs, a process in which a methyl group is covalently bonded at the cytosine 5 carbon position of a genomic CpG dinucleotide, and the association between DNA methylation and aging has been extensively studied and their close correlation has been found. DNA methylation is an epigenetic modification that plays a key role in many aging-related biological processes. Based on this, we can use DNA methylation analysis for related studies, such as age prediction, disease risk prediction, and aging mechanism exploration.

DNA Methylation Age Acceleration Age acceleration can be defined as the difference between the DNA methylation age and the actual age, or as the residual resulting from regressing the DNA methylation age on the actual age. In comparison, the latter definition is more accurate and practical. We can determine the rate of biological aging based on the specific value of the DNA methylation age acceleration, with positive values indicating faster than expected aging and negative values the opposite. DNA methylation age acceleration is closely related to the health status of the organism, aging-related diseases, mortality and other factors. On the one hand, several studies have identified DNA methylation age acceleration in age-related diseases, such as cancer, cardiovascular diseases, and neurodegenerative diseases. On the other hand, there are also some intrinsic and extrinsic factors that contribute to age acceleration in individuals, such as stress, alcohol consumption, smoking, etc.

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