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Proteins are complex molecular entities with different sizes, molecular structures, and physical and chemical properties. Protein characterization is one of the important parts of protein expression. Characterizing a protein can obtain useful information on its identity, purity, structure, conformation, and activity. The precise molecular weight, amino acid sequence, charge variances, aggregation level, glycosylation, and oxidation level are the key to the in-depth characterization of proteins. Different post-translational modifications, highly specific three-dimensional structures, and the potential for aggregation, adsorption, and truncation determine the complexity of proteins. No one analytical platform or technique can meet all the requirements to characterize proteins so far.

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In the fields of biotechnology and immunotherapy, antibodies have long been hailed as potent tools for treating various diseases, particularly cancer and autoimmune disorders. Traditionally, immunoglobulin G (IgG) antibodies have held a prominent position in this arena. However, recent breakthroughs in non-IgG antibody engineering have ushered in a new era of antibody engineering, paving the way for more effective and versatile therapies. IgG antibodies, especially IgG1, have been the preferred choice for antibody-based therapies due to their long half-life and robust effector functions. However, researchers have encountered certain limitations in IgG-based therapies, including immunogenicity and inadequate tissue penetration. To address these challenges, non-IgG platforms have emerged as a compelling alternative. One of the most promising non-IgG platforms involves using non-IgG isotypes such as IgA, IgM, and IgE, as well as smaller antibody formats like single-chain variable fr

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Fucosylation is a biological activity that is frequently seen in plants, bacteria, fungus, vertebrates, and invertebrates. It is becoming more and more accepted that the fucose moiety on the cell surface glycans is necessary for signaling and cell-to-cell interactions. Phase 1 clinical trials are now discussing the advantages of better homing and trafficking as a result of immune cell-surface fucosylation.

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