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CRISPR (clustered regularly interspaced short palindromic repeat) is a segment of nucleic acid containing short, repetitive base sequences derived from DNA fragments of bacteriophages that had previously infected prokaryotic cells. These sequences, found in prokaryotic genomes, play an important role in prokaryotic defense. The prokaryotic immune system consisting of CRISPR and Cas (CRISPR-associated) proteins now form the basis of genome editing technology. CRISPR-mediated genome editing is a rapidly expanding field. In addition to its widespread use in basic biology research such as gene knockdown or knock-in, gene repression or activation, genomic DNA tagging, and gene screening, the CRISPR-Cas system is also a powerful tool for the development of biological products and therapeutics. The core concept of synthetic biology is to construct artificial decision-making circuits by building and integrating standardized components and modules. The early stage of synthetic biology mainly

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