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Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (Tandem Fab) is designed to be expressed as three chains derived from the variable domains of anti-Ad fiber knob and anti-CD40 parental antibodies. It contains a His-tag for affinity purification. And the two Fd regions are connected by a flexible linker. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.

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Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (Single-chain Diabody) is designed to be expressed as one polypeptide chain with four variable domains from two parental antibodies. The middle linker is long enough to form scFv dimers between the two diabody chains. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.

Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (scIgG) is designed to be expressed as two chains, each chain has the same units with their parental IgG antibodies, anti-Ad fiber knob and anti-CD40 IgGs. The two chains form heterodimer via Fc KIH technology. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.

Ad fiber knob CD40 scFv IgG: Recombinant Anti-Ad fiber knob x Anti-CD40 Bispecific Antibody (scFv-IgG) is formed by an IgG and two scFv fragments. The scFvs from an anti-Ad fiber knob antibody variable domain are respectively connected to the N terminus of the anti-CD40 IgG heavy chains or light chains to form scFv-(H)IgG or scFv-(L)IgG. It is a type of appended IgGs and has 2+2 antigen-binding valency. This BsAb can retarget of adenoviral vectors to tumour cells. It can be used in cancer gene therapy to transfer a therapeutic gene into tumour cells aiming at selective and efficient cell killing.

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